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KMID : 0357319760110010079
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Journal of the Korean Society for Microbiology
1976 Volume.11 No. 1 p.79 ~ p.85
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Bacteriological Studies on Liver Abscess of Cattle by the Gas Jet Anaerobic Culture Method
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Abstract
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There are many of anaerobic culture methods and equipments for isolation and cultivation of anaerabic bacteria, but most of these methods are used without pre-reduced media. Gas-jet method is a recommendable method for the culture of anaerobes, recently developed. Bacteriological studies were experimented of liver abscess of cattle by the use of gas-jet method The results were summarized as follows;
1. Gas-jet method for anaerobic culture are expedient for the making of pre-reduced media, maintaining of oxygen free condition in the culture tube, picking of bacteria from colony and colony counting etc.
2. A 121 strains of facultative anaerobic and anaerobic bacteria were isolated from liver abscess of 27 head of cattle, and the isolated anaerobic bacteria were as follows.
Peptostreptococcus spp. 7 strains
Acid aminococcus fermentans 1
Veillonella spp. 1
Bacterioides spp. 6
Bifidobacterium spp. 4
Arachinia propionica 2
Lactobacillus spp. 4
Propionibacterium acnes 1
3. Liver abscess were infected with many of bacteria, about 103-109 numbers per gram of abscessed tissue.
Almost of abscess were mixed infection of various bacterial species rather than simple species.
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